FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16973304
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9001.072367E-77.331794746
TGGTTCG12400.00172813115.844452470
TCTCGTA13203.8140206E-75.775242341
TCCGATC8700.00123929665.56042058
CGCCTAT8850.00153199025.4679641
GATCGGA13503.6693382E-65.37683151
CACGATC14957.6120887E-75.322295736
TCGTATG12603.4522018E-55.229267643
CGTATGC12653.6585683E-55.21097244
ATCGGAA17309.409632E-85.1940882
CTCGTAT12703.9602794E-55.186083342
Produced by FastQC (version 0.11.5)