FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11183033
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGG10108.776369E-76.497077524
CCGCGAA9401.3151801E-56.24660165
CCCGCGA10156.528431E-66.1263674
AATACGC6650.0084338676.048150568
TAGCCGG9408.783632E-55.87891779
AGTACGC11751.8512692E-65.87860225
CGCGAAG9459.482649E-55.84783846
GTGCGAT8508.018264E-45.75425154
ACGCACG12354.4699827E-65.59295228
ACGCTTG8100.0031492225.556885241
CGGTACA13152.2492277E-65.522148637
GTACGCA11303.6828904E-55.50136126
CGGAATC9809.6664793E-45.2876384
CCCGTCG11201.8910979E-45.24221423
GCCGGTA12603.6469224E-55.211914535
CGCACGG14351.1302203E-55.05411929
Produced by FastQC (version 0.11.5)