FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15371065
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18100.034.92282546
CGTATGC20050.031.45410344
TCGTATG21350.029.99431643
TCTCGTA20550.029.25597441
CTCGTAT21200.029.03746442
CGATCAG22050.028.9891933
GTCACGA23250.027.01536629
ATGCCGT24250.026.8149947
ATCTCGT23050.025.90970440
GATCGGA27050.025.8980871
GCCGTCT25300.025.64020349
ACGATCA24850.025.5731732
TCACGAT25950.024.60804430
TGCCGTC25950.024.55731248
ATCGGAA29350.023.8622192
AGTCACG29150.022.37163528
CCGTCTT30900.020.91225650
CGTCTGA30350.020.69539516
CACGATC31550.020.68080931
ACACGTC31500.020.1577713
Produced by FastQC (version 0.11.5)