FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12975813
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGC8451.336312E-46.119685625
GTACGCA9409.024821E-55.86793226
GCCGGTA10456.717254E-55.615217735
TACGCAC10659.120023E-55.50286527
ACGCACG9759.283798E-45.30366628
CCGGTAC10604.91525E-45.21173436
CGGTACA9350.00308594535.171386237
CGAAATC17551.3613862E-75.10821874
Produced by FastQC (version 0.11.5)