FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12901489
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG9301.0779011E-56.32754236
GTACGCA11507.729339E-65.718907426
AGTACGC12452.9175359E-55.28254525
CCGCGAA9900.00107736095.2448765
CCCGTCG12059.8224744E-55.170681523
AACTGCG17551.14554496E-75.148185748
GCCGGTA12804.585833E-55.14023335
CCGTCGG11502.7129272E-45.116976324
TACGCAC13602.3122026E-55.09033927
AAGTACG15155.418986E-65.02657824