FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14386003
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC4750.00340357777.37249662
TTGCGTA6300.008096046.072137448
CTCGCGA7950.00251136075.663971
CGCGAAG9951.9143654E-45.5689936
TCGCGGG12900.0033056545.534141570
CCGCGAA10851.126932E-45.4262025
CGCGTCG8450.00517046275.32817521
TTACACG18152.510587E-45.1436270
GCCGGTA14954.066813E-65.10363135
Produced by FastQC (version 0.11.5)