FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14791500
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAT11400.011.02285670
GATCGGT7454.1963973E-58.80027270
GCGTCGG10252.198417E-46.9293270
GCGTCGT13209.931682E-106.64772959
CGTCGTG14156.7848305E-106.45559260
AGCGTCG13902.1198503E-86.054672758
Produced by FastQC (version 0.11.5)