FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9797255
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGC10009.6897566E-86.923824327
GCCGGTA10553.473724E-86.89674937
GTACGCA10552.5658483E-76.562833328
CGGTACA11201.07502274E-76.499015339
CCGGTAC11551.9296567E-76.300754538
ACGCACG11702.5046575E-76.214005530
AAGTACG12751.9778054E-75.97358726
GCGAATG11701.4775869E-65.953512752
TGAAGCG11350.0043754125.86502370
ACTGCGA12701.0620424E-65.751169749
TACGCAC11558.298313E-65.694803729
TGCGAAT11851.1631346E-55.581606451
CTCGAAC14001.1834054E-65.4455411
CGCACGG12101.8071072E-55.436643631
CTGCGAA15301.0542171E-65.23558952
Produced by FastQC (version 0.11.5)