FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385428
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG18050.062.57681733
GATCGGA20000.058.36761
TCGGAAT18650.057.86591337
AATCGCG4150.057.61471641
TTTCGGA19500.057.4321835
GTTTCGG19800.056.8892534
ATCGGAA20850.056.1379782
TTCGGAA19950.055.8050636
ACACGTC21550.052.6990313
CGGAATC19200.052.62234538
ACGTTTC21600.052.4428132
CACGTCT21750.052.2136714
ACGTCTG21750.051.8962615
CGTCTGA21850.051.81672716
TCGGAAG22650.051.5219573
GTCACGT21950.051.4241629
AGTCACG22100.051.38751628
TCACGTT22150.051.12021330
AATCGGG7700.050.4033941
CGGAAGA23250.050.3408624
Produced by FastQC (version 0.11.5)