FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15537871
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG7402.4505152E-67.495207343
CGCGAAA5754.214005E-47.214628736
AGTCCGC11000.00323282976.026546570
CGCGAAG15151.8405444E-75.4629066
AGTACGC19704.110916E-105.4265125
TACGCAC22301.2732926E-115.41239827
GTACGCA19955.7298166E-105.35850926
TTTGCGC11251.9287637E-45.235350642
GCGAAAT9400.0032453755.150583337
TGCGCGC12955.401289E-55.089509544
Produced by FastQC (version 0.11.5)