FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14964124
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA19500.07.094564426
AGTACGC19200.06.84521425
GCCGGTA21900.06.32210835
CGGTACA22150.06.25305337
TACGCAC23250.06.098995727
ACGCACG22650.05.802410628
GCGAATG24700.05.78717652
CCGGTAC23600.05.720971636
TGCGAAT24500.05.688243451
ACTGCGA26500.05.514702349
CTGCGAA27450.05.32723450
AAGTACG25253.6379788E-125.20509124
CGCACGG26700.05.18130229
AACTGCG29650.05.160838648
Produced by FastQC (version 0.11.5)