FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14786725
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA4354.5474735E-1113.55182736
TCCGCGA6503.4997356E-99.60031634
TATGCCG7402.3483262E-67.516512448
GCGAAAT8101.204482E-67.27916537
CGTTCGA8952.7553143E-45.80380122
TAGTCGC9253.7779313E-45.670290559
CCGCGAA13101.9956715E-65.558206635
CTCCGCG13152.1482629E-65.536003633
GATCGGA12151.1092848E-45.1308291
Produced by FastQC (version 0.11.5)