FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21632064
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA10000.025.66438536
TCCGCGA13950.018.1424134
GCGAAAT16500.015.1368437
TATGCCG13400.014.27134548
GATCGGA19550.013.8170971
ATCGGAA22200.012.0095122
TCGTATG16800.011.37092445
TCTCGTA19900.011.16357843
ATCTCGT21450.011.00179442
ACTCCGC24600.010.70841632
AATCTCG21250.010.61222641
CGAAATC23000.010.55942738
CTCGTAT18800.010.5274844
CGTATGC19100.010.18685446
CTCCGCG26400.09.71658833
CCGCGAA26900.09.6681835
CGTCTGA29900.08.91698716
ATGCCGT26100.07.996581649
CACTCCG38500.07.465953431
ACACGTC36600.07.18993813
Produced by FastQC (version 0.11.5)