FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18078757
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTT8150.00110186427.343268470
CGACGGA7850.00214870765.738744330
CGATCGG12353.724257E-65.651226557
GCCGGTA24700.05.47831735
CGGTACA27200.05.358896737
GTACGCA23509.094947E-125.308161726
TGCGAAT28200.05.18488651
AGTACGC23908.54925E-115.074327525
Produced by FastQC (version 0.11.5)