FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16134494
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG10807.868948E-97.03216556
CCGCGAA11955.873153E-86.35542965
GTACGCA17901.9099389E-105.785911626
GCGCGAG7800.00900285.494721467
GCCGGTA18402.1846063E-95.450712735
ACTGCGA21501.07320375E-105.34320149
AGTACGC17753.103014E-85.251310325
TGCGAAT21354.6929927E-105.226152451
CGGTACA19052.7932401E-85.08625337
Produced by FastQC (version 0.11.5)