FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15406092
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10606.0936145E-107.533570345
CGATAGA9202.1012966E-87.524676336
GCGATAG8503.4231198E-77.32791735
TATGCCG9058.874831E-76.919555748
GTACGCA18550.06.5176626
AGTACGC19400.06.23207325
GCCGGTA20700.06.185265535
TACGCAC20950.06.100857727
CCGGTAC21900.06.00599736
AGCGATA12104.76839E-76.004177634
GATCGGA13204.0364648E-75.7583511
CTCGTAT12404.4132303E-65.59706644
AAGTACG24150.05.57843324
CGGTACA22054.5474735E-115.338793837
ATCGGAA14402.1547712E-65.27740862
ACTGCGA25101.8189894E-125.27140649
ACGCACG23801.4551915E-115.22518428
Produced by FastQC (version 0.11.5)