FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17069869
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN192370.11269565103282282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9550.013.86478248
TATCTCG9950.012.85603941
CTCGTAT11800.011.16769144
TCGTATG12650.010.9784545
TCTCGTA12300.010.42100743
CGTATGC12500.010.288446
ATCTCGT15400.08.76394642
TGCCGTC20850.08.04440350
ATGCCGT17400.08.02192749
GCCGTCT17400.06.83783751
GATCGGA21350.06.7570731
ATCGGAA22800.06.62666562
CGTCTGA23500.06.5739316
TCGGAAG28550.06.1337313
CCGTCTT22150.06.012211352
ACACGTC26550.05.81871613
CACGTCT31600.05.32338814
ACGTCTG31400.05.24796315
GTATGCC29000.05.159701347
Produced by FastQC (version 0.11.5)