FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15135869
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10400.021.09624748
CTCGTAT12250.018.66708844
CGTATGC12850.017.56847446
TATCTCG12450.017.47791741
TCTCGTA13500.017.43181843
TCGTATG13600.016.8333245
ATGCCGT18200.013.02971149
ATCTCGT18150.012.95268742
GCCGTCT19600.011.77905451
TGCCGTC21200.010.87299550
GATCGGA22200.010.8290641
CCGTCTT22100.09.98775752
ATCGGAA24400.09.7083562
CGTCTGA24450.09.54606916
ACACGTC25350.09.07178613
TCGGAAG27900.08.8594073
GTATGCC27200.08.56579547
ACGTCTG29900.08.49480915
CGTCTTC25450.08.41138253
CACGTCT30300.07.816270414
Produced by FastQC (version 0.11.5)