FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005687767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68577509
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGA318550.0130.842049
ATCGTGA279550.0129.515479
ATCATGA695750.0129.440729
GCGATGA178150.0128.923469
AGACTGA442400.0128.577749
ACGATGA451900.0128.505839
ACGGTGA50100.0127.922569
TCGATGA250400.0127.8578349
TCGTTGA185550.0127.728149
CCGATGA218800.0127.7018059
CCAATGA531550.0127.635519
AACGTGA407300.0127.3228769
ACGCTGA218950.0126.422279
CGAATGA386150.0125.96059
CCTATGA285400.0125.64149
CCGTTGA105200.0125.564039
GGATTGA231700.0124.534499
GCGTTGA130400.0124.427139
CAACTGA402600.0124.01929
CGACTGA177750.0122.238999
Produced by FastQC (version 0.11.5)