FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005687774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66264525
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATGA683800.0127.312049
ACGATGA422400.0126.872049
ACGTTGA310450.0126.734859
AACGTGA377650.0126.356129
ATCGTGA277950.0126.3471159
CCAATGA482250.0125.753949
GCGATGA169300.0125.512449
CGAATGA364550.0124.214649
CCGATGA207700.0123.669499
TCGATGA239050.0122.857439
AGACTGA445200.0122.800949
CAACTGA372600.0121.842319
TCGTTGA184750.0121.530329
CCTATGA284450.0121.293559
ACGCTGA213900.0120.1865549
GCGTTGA135750.0118.560789
GGATTGA235350.0118.289159
CGTATGA151150.0117.89679
CCGTTGA101700.0117.622639
ACGGTGA49200.0116.557259
Produced by FastQC (version 0.11.5)