FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16761166
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8500.012.26346246
CGTATGC12050.09.78136844
GTCGGCG7150.00157742197.97333870
TCGTATG13050.07.960458843
TCTCGTA11701.0913936E-117.67889641
CTCGTAT12704.134563E-96.536411342
GATCGGA20150.06.3071381
ATCTCGT15803.2196112E-106.118324840
TGCCGTC17153.110472E-105.890721348
GCCGTCT17253.4924597E-105.86449849
CGTCTGA21250.05.815935616
ATCGGAA21300.05.80349642
ATGCCGT17001.5970727E-95.729980547
CCGTCTT19501.2842065E-95.374070650
GGACCGT11051.6777264E-45.28435851
ACACGTC23501.2732926E-115.259106613
TCGGAAG26101.8189894E-125.13073
Produced by FastQC (version 0.11.5)