FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18446502
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGA344750.0131.558729
ATCATGA193200.0131.33739
ACATTGA215800.0130.483299
AATATGA287500.0129.790669
ACAATGA290950.0129.32319
ATTATGA221000.0129.053339
ACGATGA115450.0127.413449
AGTATGA99550.0127.008379
ACGTTGA83200.0126.523279
AGATTGA138250.0126.485089
GTATTGA80150.0125.277589
CGATTGA68600.0125.233769
AACGTGA104550.0125.1642469
TAAATGA287900.0125.127999
TAATTGA196000.0124.911429
GAACTGA113550.0124.565359
AATTTGA168550.0124.5652549
CGAATGA98550.0124.5453349
TCGATGA65700.0124.361429
ATCGTGA80400.0124.166679
Produced by FastQC (version 0.11.5)