FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005687789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005687789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20057666
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN237530.11842354938007243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA2450.025.3013841
ATCGCGT3250.022.22313740
CGCGTAT2950.021.04327242
TATGCCG11150.014.63109546
CATCGCG7500.013.741075539
CGTATGC14650.010.62547944
CGTCTGA34150.09.89275116
ATCGGAA36350.09.4836892
GATCGGA35300.09.478621
CTCGTAT12750.08.92619942
ACACGTC39850.08.56340313
ACGTCTG44700.07.939595715
TCGGAAG46200.07.75707343
CACGTCT45900.07.509003614
TCGTATG14800.07.46769943
TCTCGTA15800.07.41070741
ATCTCGT19400.07.0913340
CGGAAGA51500.06.8924994
TCATCGC19550.06.84594838
ATGCCGT19950.06.798585447
Produced by FastQC (version 0.11.5)