FastQCFastQC Report
Sat 28 May 2022
EGAF00005688039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24353846
Sequences flagged as poor quality0
Sequence length35-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCG1565100.642650035645294No Hit
GGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT1213940.4984592577287382No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGT1068580.4387725864736108No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG1039730.4269264082560102No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAAT884890.3633471280059831No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACG701990.28824605362126376No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG667620.2741332929509368No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG598190.24562444880369202No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG531360.21818319784070248No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT453460.1861964635893649No Hit
CAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATT398420.16359633710420934No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTT396910.162976311831815No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT392190.1610382195896287No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT348170.14296304575466234No Hit
ACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT346660.14234302048226796No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG306240.12574605259473187No Hit
GTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGG302840.12434996919993664No Hit
GTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTT295080.12116361415769814No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG293310.12043682956687826No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGT290920.11945546506288987No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATT289790.11899147264050204No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCAT288370.11840840251679345No Hit
GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTG270990.11127195269281082No Hit
CTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGG267230.10972804870327257No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGG263930.10837302658479486No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG259740.10665255910709134No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGG257740.1058313335807412No Hit
GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGG254000.10429564184646646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)