FastQCFastQC Report
Sat 28 May 2022
EGAF00005688337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69149294
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTATG10205891.4759210701413668TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCGGAACTCCAGTCACGGACTTGGATCTCGTATG4675170.6760980090411335TruSeq Adapter, Index 11 (97% over 35bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG1330530.19241411199368139No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1178110.1703719491337106No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1173810.16975010619775813No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC993270.1436413797659308No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG973320.1407563177723839No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC960640.1389226041845055No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG954230.1379956243660275No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT909820.131573288369365No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC791010.11439162343436218No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT778980.11265190936005796No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCGCGTATG777450.11243064896656789TruSeq Adapter, Index 4 (97% over 37bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA754810.10915657360146004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)