FastQCFastQC Report
Wed 1 Jun 2022
EGAF00005688342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18508156
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC3388261.8306848072817195No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1625080.8780345270485077No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG1174940.6348228316208271No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG965220.5215106248293996No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT922310.4983262514104593No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC848840.45863023847432455No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC782320.4226893268027349No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT568460.307140268322787No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGTT546960.29552376800800684No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA542380.2930491832897886No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC540550.2920604300071817No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTG499380.2698161826602283No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTG467940.25282907708363817No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCC427550.23100626556205817No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC401040.21668285052276415No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATG354530.19155338867902347No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCG345920.18690138552970917No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCC341420.18447002499870868No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG335250.18113635955953689No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC329360.17795397877562735No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGG327100.17673289548672488No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA326770.1765545957144515No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGG320660.17325334841569306No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTT317110.17133527510790378No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTC310500.16776387663903417No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGG308930.1669156019648851No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTT294720.15923790571032576No Hit
AAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCC294210.15896235151681237No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCC294120.15891372430619236No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGT291040.15724959309830758No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG289280.15629866097951625No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTT286470.15478041140349152No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGAT284120.15351070090396904No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCC281890.15230582668527323No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC278350.15039315640088619No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGC273950.1480158261039079No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGAT266980.14424991879255827No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT265050.14320713527592915No Hit
AGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGCCC258290.1395546914560262No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAT256910.1388090742265194No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCG253760.13710712185481905No Hit
CCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT253420.13692341905914343No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA252630.1364965802103678No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT247770.13387071083688726No Hit
CTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCC246510.13318992988820713No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC245100.13242810358849363No Hit
CTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCG244500.13210392218436023No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCG242600.1310773477379378No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTT237850.12851091162188175No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA232590.12566892131231225No Hit
CTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT231070.12484766175517431No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA230770.12468557105310761No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTA225010.12157342957342697No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT221120.11947165346996211No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCG219540.11861797577241082No Hit
CACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGAC217930.11774808900465288No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTT217570.11755358016217284No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT216710.11708892014958161No Hit
GTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC215990.11669990246462153No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATG214370.11582461267346136No Hit
CTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGAT209300.11308527980853414No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC209260.11306366771492524No Hit
CCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCC207670.11220458699397173No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAAC206130.11137252139002934No Hit
CGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG203160.10976782343956903No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCG201490.10886551853139773No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAG199970.10804425897425979No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAG199090.10756879291486414No Hit
ATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTAC198220.10709872987887069No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTAT197480.10669890614710617No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGC196440.10613699171327495No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCC194110.10487808726055692No Hit
ACTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC192230.10386231886093891No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATC191530.1034841072227833No Hit
TGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGT190170.10274929604008093No Hit
CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCG189250.10225221788707638No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC189130.10218738160624971No Hit
CTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGC188060.10160925810221182No Hit
CGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCAT188020.10158764600860291No Hit
CCTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCC187130.10110677692580503No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG186880.10097170134074945No Hit
CGCAGACCCGACGCACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATG185650.10030712946227599No Hit
GGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATC185500.10022608411124263No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTG185490.10022068108784042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)