FastQCFastQC Report
Sat 28 May 2022
EGAF00005688356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20171163
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC3662971.8159438798843675No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC1388350.6882845575141106No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1330580.6596446620356No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA888740.44059928522713343No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA858100.42540928354007157No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC668770.33154756619635667No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG636020.315311516742986No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA503960.249841816260173No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTC486840.24135445239325068No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGAT486340.24110657377564199No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG482210.23905909639419404No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGG477260.23660509807986776No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGAT460660.22837552797525854No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCG447050.2216282720039494No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGTT427150.21176270302312267No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC418110.20728105761675716No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGG410600.2035579207802743No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC393840.19524902951803025No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC371600.18422338860679474No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT359370.17816027762008566No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT357980.17747117506313345No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG353880.175438570398742No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTT352780.17489323744000285No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATC349370.17320270526791143No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC347800.17242436640862005No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT344120.17059997978301994No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTG340630.16886978703211114No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGG336960.1670503579788632No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCC332660.16491860186742827No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGC327870.1625439247107368No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTAT323800.16052619276340188No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCC317760.15753181906268865No Hit
ACTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC312280.15481506941369716No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGG307060.15222721664586222No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT296160.1468234627819923No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC291780.14465204609173998No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCC286500.14203444788979197No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTG285820.14169733296984413No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAT284480.14103301827465278No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTT283750.14067111549294406No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA277560.1376023782069482No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT276680.13716611183995686No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG275880.13676950605178295No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCC275170.13641751841477856No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACG274670.13616963979716984No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTG270170.13393873223869146No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCC268690.13320501153056966No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCG265810.13177723069314348No Hit
GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGC262360.13006686823164337No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCC261690.1297347108840477No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT259990.12889192358417806No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC259320.12855976623658238No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTC249700.12379058163379077No Hit
GTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC248090.12299241248509073No Hit
CTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGAT246310.12210996460640372No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG243120.12052849902606012No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTT241360.11965596629207745No Hit
GTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACC238630.11830254903993388No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAAC238240.1181092037181991No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGT234330.11617079292849895No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTT231260.11464881821638148No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC229420.11373662490358143No Hit
CAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAA226830.11245261366436829No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA225120.1116048687921465No Hit
CACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGAC224820.11145614162158127No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATG224510.11130245687866387No Hit
GGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCG223070.11058856645995076No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGG221580.10984988817947682No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACG220550.10933925822720286No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCG216640.10740084743750274No Hit
GTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCG216220.10719262939871141No Hit
GACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCG216030.10709843552402011No Hit
CCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTA213830.10600776960654178No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC211620.10491214611671126No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC210140.10417842540858947No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG209480.10385122563334598No Hit
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGGC208160.10319682608285898No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGT205150.10170459680485452No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC204340.10130303344432844No Hit
GATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCC202490.10038588255917619No Hit
ATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTAC202390.10033630683565443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)