FastQCFastQC Report
Sat 28 May 2022
EGAF00005688417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30493044
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACGGACATCTCGTATG527650.1730394643447207TruSeq Adapter, Index 23 (97% over 37bp)
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG359810.11799740294868562No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT316090.10365970678427514No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG315680.10352524988977814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)