FastQCFastQC Report
Sat 28 May 2022
EGAF00005688457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24840777
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGTATG1293871552.08659535891329TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCGCGTATG13459415.41827254437331TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGTTTG4689941.8880005242992197TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGGATG4607591.8548493873601457TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGTATT1145550.4611570725022007TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCGCGTTTG802240.32295286093506653TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCGCGGATG558740.22492855195310515TruSeq Adapter, Index 15 (97% over 38bp)
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA418150.16833209363781174No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG321190.12929949815982006No Hit
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGTATG317360.12775767843332758TruSeq Adapter, Index 1 (97% over 34bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC311420.1253664488836239No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGGTTG307320.12371593690487218TruSeq Adapter, Index 15 (97% over 38bp)
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA289350.11648186367117261No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC282340.11365989075140444No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC278550.11213417358080224No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC273090.10993617470178166No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT270800.10901430337706425No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCAT251830.10137766624610817No Hit
ACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGC250220.10072953837152517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)