FastQCFastQC Report
Wed 1 Jun 2022
EGAF00005688515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19855665
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA2730311.3750785984755483No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTG2417971.2177733659386376No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC2138371.076957130370602No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG1728870.870718759608404No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT1403210.7067051141324151No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCT1243150.6260933592503701No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC1159650.5840398697298731No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGAT1092580.5502610967701158No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT1018250.5128259365777977No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGA990990.49909685724451935No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCAC972410.4897393262829525No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT876370.44137025881530534No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAA720250.3627428242771018No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGG716000.36060237720569926No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAA671860.3383719457394149No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG659450.3321218402909195No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG654240.3294979039986825No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA652250.3284956711346611No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTC614340.3094028832577504No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT609270.3068494558102184No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTC594250.2992848640425793No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGAC590370.29733076177504No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATT570980.2875652867833941No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG561300.2826901038066466No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGG554650.27934093368315793No Hit
GCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGT520960.2623734838394987No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCC518170.2609683432914486No Hit
GGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTC506190.2549348007231186No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGT499590.2516108123298817No Hit
GGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGA492860.24822135143799012No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGC474690.23907031066448792No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCC451280.22728022456059768No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGT449060.226162155737418No Hit
GGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGG429830.2164772622825778No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCG424090.21358639964967177No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGAC422330.2127000027448086No Hit
GCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGG422270.21266978466850645No Hit
GCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTC412460.20772912919310432No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGG409520.20624844345429882No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGC405020.2039820877316373No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT403320.2031259089030763No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA394150.19850757957489712No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCAT388990.1959088250129119No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCC383630.19320934352991956No Hit
TGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA380730.19174880317531545No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGAC373020.18786578037048873No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCT370380.18653618501319397No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC369620.18615342271336668No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG367180.18492455427707913No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC365370.18401297564196414No Hit
GTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCG365190.18392232141305767No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGG365120.18388706699070517No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAG355490.17903706574420952No Hit
GTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACG346200.17435830026342608No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTC344320.1734114672059586No Hit
GTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCC343650.17307403202058455No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGC336760.16960398959188727No Hit
GTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGAT336640.16954355343928293No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC328380.16538353160168648No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGG323580.16296608549751418No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGA322370.16235668762542074No Hit
GTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTG317550.15992916882914776No Hit
GCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGA316830.1595665519135219No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAGA304650.15343228242418475No Hit
GGTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG303390.15279770282183952No Hit
GTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGG302040.15211779610504106No Hit
GCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGTC294570.14835564560542294No Hit
GTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCAT293210.1476707025425741No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGC291240.14667854237065342No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGG289220.1456612004684809No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGT286710.1443970776098408No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACG286200.14414022396127252No Hit
AGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAG285420.1437473889693445No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGC285300.1436869528167402No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG283310.14268471995271878No Hit
GGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTG278650.14033778269325153No Hit
GTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAA276130.13906862348856106No Hit
GGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG275460.13873118830318704No Hit
GTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCC264370.13314588053333898No Hit
GCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAG263520.13271779111905846No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGT259060.1304715807805984No Hit
GTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTC258290.13008378213472074No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGGGTGGA254790.12832106101709512No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCT251940.12688570239274283No Hit
GTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACC251090.12645761297846234No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGA250280.12604966894838324No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG249460.12563668857225382No Hit
GGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACG249060.12543523473023946No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCA248450.12512801762116757No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTA248400.12510283589091578No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGG245930.12385885841647712No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG244530.12315376996942687No Hit
GGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA240160.12095288674542001No Hit
GTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGG238190.1199607265734993No Hit
GCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC235240.11847500448864343No Hit
GCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG231520.1166014837579099No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGAACA227470.11456176360751452No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCC224460.11304582344635648No Hit
GCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGG220380.11099099425781005No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG220300.11095070348940718No Hit
GTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACGAGCGCACGTGTTAGGACC217000.10928870929278872No Hit
CCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAG216330.10895127410741469No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGG215960.1087649293035514No Hit
GCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAAC215930.10874982026540032No Hit
GGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC215150.10835698527347233No Hit
GTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTC214000.10777780547768107No Hit
GGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCG211160.10634748319937912No Hit
GTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTAT210410.10596975724560219No Hit
GGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTA208920.10521934168409872No Hit
GCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGG206670.10408616382276796No Hit
GCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTATGACT206240.10386960094260253No Hit
GTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTA205380.10343647518227166No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAG205260.10337603902966735No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGG204440.10296305865353791No Hit
GGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGA203000.10223782482228624No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAG202400.1019356440592647No Hit
GTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGA201970.10171908117909925No Hit
GGTGGAGCCGCCGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAAC201640.10155288175943743No Hit
GTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAG199870.1006614485085239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)