FastQCFastQC Report
Wed 1 Jun 2022
EGAF00005688529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24128223
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC2283980.946600999170142No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1923570.797228208641805No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACG1888580.7827265190644169No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT1575260.6528702921885295No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG1516140.6283678661292214No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG1433020.5939185824003699No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCG1367610.5668092507268355No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC1366430.5663201968914163No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGT1363150.5649607930099121No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC1297020.5375530556062914No Hit
GGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT1278390.5298318073403084No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG1218310.5049315069742185No Hit
GTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCA1202120.49822152257130586No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCC1046140.43357523676733256No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC940100.38962670396406734No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG932300.38639397522146574No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTG919590.38112628518063685No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCC859010.35601875861309806No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC850410.35245446794817836No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC802340.332531740940889No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG777890.3223983796900418No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG773490.32057478911729226No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGC742630.3077847879638712No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT735240.30472198470645767No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAAT728500.3019285755109276No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT718850.29792910982296544No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTT715950.29672719785456225No Hit
GATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGC707350.2931629071896426No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTG664850.2755486800664931No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT664510.2754077662495079No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGC656810.27221648274719606No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT656060.2719056434450228No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATT653860.2709938481586481No Hit
GTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGG649100.26902105472085536No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG648420.2687392270868849No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC631790.2618468836266972No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCG612800.25397643249567114No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC610290.2529361569643981No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCC605460.2509343518584025No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT593940.24615986017702177No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCC584090.24207750400848No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCT581070.24082585775172918No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCT556930.23082097674578025No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCG547820.22704531535538275No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC533010.2209072752684688No Hit
GTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTG526790.2183293813224455No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA525400.2177532924824178No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC517430.21445010683132362No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCT510070.21139973714599702No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG489790.202994642415233No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA483410.20035043608474606No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTG482640.2000313077345149No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGC482620.20002301868645694No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT476780.19760261665353474No Hit
GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTG474180.19652504040600088No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTCT468060.19398859170026736No Hit
ACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTC467510.19376064287867364No Hit
GTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCC459450.19042015651131872No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACC456870.18935086931184283No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACC456460.1891809438266548No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCC456060.18901516286549574No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCC455810.1889115497647713No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC453500.18795416471407778No Hit
GTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCATCGGG452680.18761431374370174No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT449470.18628392153040033No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT446170.18491622860083812No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGT445220.18452249881808536No Hit
GTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCG444720.18431527261663652No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATC443740.18390910926179685No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTC442660.18346150066666742No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC442430.18336617661400095No Hit
GTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCAT436840.18104938768180318No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC433420.17963196046389326No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCC429860.17815650990957765No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCC429340.1779409946600709No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATT429180.17787468227560727No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCC426670.1768344067443342No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCC419030.1736679903861963No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA416870.1727727731959374No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATT415840.17234588722095284No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTG401410.16636533904713993No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT401380.166352905475053No Hit
GGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGG400380.16593845307215538No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAGT399660.16564004734206908No Hit
GTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGAT399560.16559860210177932No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCT399460.16555715686148956No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCT397070.1645666156185642No Hit
GTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCC396280.16423919822027508No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTG389990.16163229260604894No Hit
GGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCC389020.16123027377523824No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAAC386770.16029775586871856No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC381780.15822963837825935No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC377940.15663814115113242No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT377770.15656768424263984No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAC366150.1517517473209693No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCC358730.14867651049146885No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCC353670.1465793813328068No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCC352720.14618565155005406No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGG352530.1461069055935035No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG349910.1450210402979117No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCG349790.14497130600956398No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCAC349680.14492571624524525No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTAC348070.14425844787658004No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTT345360.14313528186472746No Hit
CCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC341750.141639108690267No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCT333710.13830691137097No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACT321940.13342880658886483No Hit
CAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCG313260.12983135973171336No Hit
GCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCC312140.129367173040468No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGC310860.12883667396475904No Hit
ATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCG309530.12828545226890517No Hit
GATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTC308980.12805750344731148No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT308860.12800776915896378No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC306380.1269799271997776No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC304830.12633752597528627No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATT304190.1260722764374318No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT303140.1256371014143893No Hit
GTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCC302920.1255459218857518No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTAC296640.12294316079555466No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCT293000.12143455404900726No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT292580.12126048403979024No Hit
GCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGAC292580.12126048403979024No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA290450.12037770042161829No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC287230.1190431636842879No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACC287020.1189561286796794No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTT286990.11894369510759246No Hit
CTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCTGC285150.11818110268626081No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGG282790.11720299501542239No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGC281590.11670565213194523No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTC280790.11637409020962713No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGG279340.11577313422542555No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT277320.11493594037157234No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA277080.11483647179487691No Hit
ACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGT276400.1145546441609065No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG274300.11368429411482146No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCC272300.1128553893090262No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT271960.11271447549204101No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC270740.11220884356050588No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA269920.11186899259012983No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTC267310.110787271818567No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC265030.10984232033996039No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC262710.10888079076523786No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCAT261610.10842489312205048No Hit
CCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGGAGACCT261430.1083502916895289No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGT261220.10826325668492039No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTC261160.10823838954074654No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT258780.10725199282185018No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC258380.1070862118606911No Hit
GTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACC258070.10695773161579283No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC255450.10587186632020104No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGC254580.1055112927296801No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC253860.1052128869995938No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCAT253750.10516729723527507No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC253460.10504710603843473No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCT253340.10499737175008701No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCT252810.10477771197655128No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAC248410.10295412140380168No Hit
CACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGC247890.1027386061542949No Hit
GCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTT246890.10232415375139728No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG246440.10213765017009335No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCATC246040.10197186920893428No Hit
GGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC245000.10154083870992076No Hit
CCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATC242810.10063318794757492No Hit
GGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTT241940.10027261435705398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)