FastQCFastQC Report
Sat 28 May 2022
EGAF00005688542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15107559
Sequences flagged as poor quality0
Sequence length35-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC3342032.2121575034060768No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1181800.7822574116705419No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG995300.6588092755421309No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC780360.5165361260545135No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC565490.37430931098796305No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA561820.3718800634834522No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGTT400200.2649005044428422No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTG378010.2502124929646146No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTG345420.22864051035643812No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT345370.2286074143413903No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA329670.21821526561637125No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA318870.21106652636603968No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCC302580.2002838446634562No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTT289120.19137439741258003No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC288630.19105005646511125No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAT279800.18520530020766426No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGG278860.18458309512476503No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTC271320.17959221605555206No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTT270870.17929435192012158No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTA267760.17723577978414645No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC266550.17643485621998894No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGT257530.1704643351053602No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGAT251710.1666119589537926No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG251700.16660533975078304No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGAT239390.15845710084600695No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG236000.156213191025764No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT235510.15588885007829525No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTT233410.15449881744628632No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT231440.15319483445340176No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTT227990.1509112094151014No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA227680.15070601412180484No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCG227400.150520676437537No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCC221430.1465690122408259No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGG220710.14609242962413715No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTT216300.14317336109691844No Hit
CACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGAC215180.142432010359847No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG203680.1348199268988458No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGG202200.13384028485343No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCT199410.131993527213761No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCC199080.1317750935144453No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC198420.13133822611581394No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTG193710.12822058149830823No Hit
CGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCAT192450.1273865619191029No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC191500.1267577376331941No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTAT188560.1248116919483816No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCC188250.12460649665508505No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGC187710.12424905969256846No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTG186350.12334884808326745No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGC185610.12285902706055955No Hit
CTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCG185300.12265383176726301No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATG184820.12233611002280381No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATC184140.1218860042181533No Hit
CCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT183370.12137632558641671No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC183120.12121084551117754No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCG182310.12067469006740268No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTG181910.12040992194702003No Hit
CGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGG179730.11896693569093458No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG178730.11830501538997795No Hit
CTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCT175050.11586914868245758No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAG173350.11474388417083131No Hit
CCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG172930.11446587764442952No Hit
CCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTG171920.11379733814046333No Hit
CTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCG171540.11354580842609982No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAAC170150.1126257392077701No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCG170120.11260588159874141No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGAC167610.11094446164334026No Hit
CTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGAT166970.11052083265072803No Hit
ACTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC165840.10977286271064704No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT165820.1097596243046279No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAGC162920.10784005543185368No Hit
CCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTA159930.10586091373199337No Hit
CGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCG159770.10575500648384031No Hit
CTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCT158670.10502689415278803No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCG158420.10486141407754887No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGC158400.10484817567152974No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA157820.10446426189697489No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCC157550.10428554341571661No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACG156450.1035574310846643No Hit
CAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCT156190.10338533180641558No Hit
CTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCT155930.10321323252816686No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC154650.10236597454294238No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT154360.10217401765566497No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT154290.10212768323459799No Hit
CTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGC152830.10116127959520131No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGT152340.10083693864773256No Hit
CCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT152180.10073103139957951No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAG151120.10002939588056549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)