FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7283254
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA195000.011.562604145
CACGTCT245400.011.403616145
ACGTCTG217700.011.222803145
ACACGTC248800.010.723272145
CACACGT274450.010.619222145
GCACACG303650.010.123308145
GATCGGA566350.09.139937145
CGGAAGA500500.09.082248145
GAGCACA403400.08.98595145
AGAGCAC416450.08.965494145
TATGCCG13854.8301445E-88.89875145
CACACTA76600.08.8967145
TCGGAAG516300.08.818352145
ATCGGAA540200.08.723458145
GAACTCC201750.08.696244145
AGATCGG606650.08.688126145
AGCACAC367850.08.553598145
TCTGAAC227650.08.503025145
GTCTGAA243500.08.455694145
CTGAACT231800.08.350793145
Produced by FastQC (version 0.11.5)