FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5259542
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG10600.041.034107145
CCGTATC39700.013.147452145
CGCCGTA40450.013.082898145
TGGTCGC66450.012.764109145
GTGGTCG76250.012.739857145
GATCTCG156800.012.113088145
GCCGTAT40100.011.931614145
CGTATCA34900.011.424492145
TCGCCGT57750.011.423233145
CGGTGGT100250.011.208486145
GTGTAGA236100.010.746615145
GAGTGTA262950.010.669336145
AGATCTC183950.010.640538145
AGTGTAG247200.010.615971145
AGAGTGT299950.010.344143145
TCGGTGG119500.010.312897145
TCTCGGT126050.010.179584145
GTAGATC254450.09.458782145
TCGCGTA1400.00132535979.3217745140-144
TGTAGAT250150.09.244636145
Produced by FastQC (version 0.11.5)