FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10691359
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT112000.016.117489145
TATGCCG69850.015.256924145
ATCTCGT135600.014.435111145
CGTATGC88000.013.757828145
CATCTCG174400.013.426786145
TAGCCAT275850.012.50979145
TCGTATG97500.012.491676145
TCTCGTA122250.012.334754145
TGTAGCC351100.011.129457145
GTAGCCA342700.010.809929145
CTGTAGC394450.010.75177145
CCTGTAG470700.010.719662145
GTATGCC104700.010.524778145
ATGCCGT81600.010.483539145
GTCACCT585800.010.358387145
AGTCACC630050.010.3097725145
TCCAGTC799100.09.934113145
CCAGTCA744800.09.772599145
ACCTGTA528700.09.598532145
CACCTGT606050.09.426139145
Produced by FastQC (version 0.11.5)