FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10691359
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG23450.038.33341145
GTGGTCG163800.015.401533145
TGGTCGC141300.014.929643145
CGCCGTA79800.014.535022145
GCCGTAT76400.013.948342145
GTCGCCG129400.013.893639145
CCGTATC76250.013.785635145
TCTCGGT290400.012.581503145
CGTATCA67200.012.190114145
CTCGGTG298850.012.1529875145
CGGTGGT221750.012.030475145
AGATCTC424900.011.959965145
GATCTCG365400.011.248979145
TAGATCT442200.011.213366145
GGTCGCC147850.011.130203145
AGTGTAG594000.010.922831145
GGTGGTC254000.010.788391145
GTGTAGA568100.010.770014145
GTAGATC601400.010.511185145
TCGGTGG262250.010.50429145
Produced by FastQC (version 0.11.5)