FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8002271
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG19800.027.830109145
CGCCGTA39150.015.186181145
TCGCGTA3951.3368288E-512.848945145
CGGTGGT111300.011.856124145
TAGATCT220150.011.23058145
GCCGTAT39750.011.126517145
TCTCGGT144500.010.687552145
TCGGTGG145350.010.625053145
AGATCTC215100.010.449314145
CCGTATC40350.010.421998145
GAGTGTA302550.010.304759145
GTGTAGA276400.010.282875145
CGTATCA36900.010.217473145
GATCTCG170250.010.178348145
AGTGTAG283850.09.961901145
GTGGTCG80400.09.919806145
GTCGCCG69850.09.653461145
AGAGTGT345500.09.653312145
TAGGGAA531250.09.621808145
AAGAGTG419850.09.5498705145
Produced by FastQC (version 0.11.5)