FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9669821
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG21300.031.991888145
CGCCGTA46050.019.677595145
GCCGTAT45000.016.270485145
TGGTCGC89900.014.192033145
GATCTCG234500.013.571045145
GTGGTCG102200.013.406102145
GGTCGCC94650.012.637319145
GAGTGTA428550.012.33155145
TCTCGGT183000.012.121656145
AGTGTAG398150.011.871142145
GTGTAGA382850.011.758572145
TAGATCT296200.011.576245145
AGATCTC284850.011.477623145
AGAGTGT502600.011.380101145
CGTATCA41550.011.340546145
TCGGTGG174750.010.993105145
GTCGCCG76400.010.911793145
TGTAGAT406750.010.586454145
GTAGATC419250.010.564764145
TCGCCGT68550.010.469341145
Produced by FastQC (version 0.11.5)