FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556068
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37450.013.744779145
ATCTCGT64250.012.525096145
ACGGTGC167600.011.722669145
CGTATGC47050.011.710763145
GTGCGAA122300.011.618789145
TGCGAAA112150.011.571376145
GTCACGG205450.010.974519145
GGTGCGA140900.010.908276145
CTCGTAT56600.010.887639145
TCGTATG52200.010.694278145
CGAAATC96650.010.351623145
TCTCGTA59850.010.296413145
CGGTGCG145250.010.282111145
TCACGGT199200.010.117814145
AGTCACG221400.09.954678145
GCGAAAT98600.09.411703140-144
AACTCCA404050.09.168896145
CGTCTGA503800.08.950833145
TCCAGTC326000.08.739894145
CTCCAGT362400.08.702265145
Produced by FastQC (version 0.11.5)