FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6965393
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG18150.027.162622145
CGCCGTA61250.015.506172145
CGTATCA47750.014.575963145
GCCGTAT57250.013.930177145
TGGTCGC108950.013.9078145
GTCGCCG99600.013.102452145
CGGTGGT168250.012.927233145
GTGGTCG126950.012.678259145
TCTCGGT208800.011.944484145
TCGCCGT86100.011.87286145
GATCTCG267850.011.774353145
CCGTATC56100.011.50182145
TAGATCT301150.011.098325145
AGATCTC302150.010.869637145
GGTCGCC116250.010.726916145
AGTGTAG397500.010.542172145
AGAGCGG41000.010.25613145
CTCGGTG227900.010.211748145
GTGTAGA380050.09.91978145
GAGTGTA425500.09.882523145
Produced by FastQC (version 0.11.5)