FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8814352
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG20950.024.22437145
CGCCGTA32850.012.3592205145
GAGTGTA234450.011.039679145
AGTGTAG225750.010.662248145
GATCTCG140550.010.007124145
GTAGATC236300.09.971444145
TGTAGGG476200.09.880837145
GTAGGGA446950.09.781305145
GTGTAGA220100.09.750124145
TCGTGTA542750.09.644412145
GTGTAGG500550.09.61743145
GTGGTCG72400.09.51313145
CGTGTAG513400.09.503808145
TGTAGAT239650.09.4690275145
TCTCGGT110050.09.354849145
TAGGGAA425100.09.260771145
GCCGTAT33700.09.250751145
CGTATCA32950.09.241283145
CGTCGTG626700.09.150719145
AAGAGTG333200.09.073389145
Produced by FastQC (version 0.11.5)