FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7402857
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27100.014.178298145
TCTCGTA46100.011.637195145
CTCGTAT40850.011.5355625145
CGTATGC32950.011.441038145
ATCTCGT48000.011.327591145
TCGTATG37450.010.647028145
TCCAGTC302250.010.193895145
GTCACAC209350.010.007887145
ACGTCTG526200.09.754385145
CGTCTGA483400.09.568229145
CCAGTCA284950.09.540697145
ACACGTC590250.09.273174145
CAGTCAC286000.09.252187145
AACTCCA386300.09.252088145
TGAACTC438050.09.118963145
TCTGAAC479700.09.052627145
CACGTCT571850.09.013739145
GTCTGAA513600.09.00561145
CACACGT637650.08.993145145
CTCCAGT350650.08.952237145
Produced by FastQC (version 0.11.5)