FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7344289
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC72600.017.375084145
TCGTATG83800.016.956114145
TATGCCG56550.015.511972145
CTCGTAT93900.015.209498145
TCTCGTA103250.014.042815145
ATCTCGT112850.013.683345145
ATGCCGT68150.011.914243145
ACCAATG360350.011.628335145
GTATGCC80100.011.222857145
CACCAAT383150.011.144503145
AATCTCG174700.010.955325145
GGAAATC228100.010.90142145
CCAATGG336950.010.886776145
CAATGGA323400.010.872164145
AAATCTC198850.010.463346145
AGTCACC493900.010.348189145
CCGTCTT64750.010.300594145
GTCACCA494700.010.258182145
CCAGTCA582900.010.049201145
TCACCAA439650.09.811246145
Produced by FastQC (version 0.11.5)