FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5880337
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG11450.024.060827145
TGGTCGC43700.013.438044145
GTGGTCG50600.012.895091145
GCCGTAT21950.012.220799145
AGTGTAG167000.011.981882145
GAGTGTA181950.011.116923145
GTCGCCG38700.010.865493145
CGCCGTA21450.010.815712145
GTGTAGA163050.010.3157215145
GGTCGCC51600.010.116149145
AAGAGTG262900.010.037909145
GTGTAGG388150.09.974109145
CCGTATC24100.09.9272585145
GTAGGGA342700.09.710263145
TAGGGAA325400.09.669513145
AGAGTGT211600.09.661974145
TAGATCT132550.09.626432145
GATCTCG109100.09.569079145
TGTAGGG366600.09.512289145
CGTGTAG398650.09.4204235145
Produced by FastQC (version 0.11.5)