FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8702354
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT257650.012.352283145
AGTCACG278600.012.334174145
GTCGGTA4150.002818189612.2300342
TCACGTC254050.012.213423145
TCTCGTA67850.011.860035145
CGTCTGT191350.011.403833145
CGTATGC50350.011.230729145
CTCGTAT61850.010.783529145
TCCAGTC436150.010.737656145
ATCTCGT72850.010.647975145
CGTCTGA711250.010.162152145
ACGTCTG936750.010.0608145
TCGTATG57350.09.859934145
CAGTCAC393550.09.781545145
GTCTGTC217400.09.770593145
GAACTCC579400.09.684458145
TGAACTC618850.09.629401145
CACGTCT1012800.09.505768145
AACTCCA542300.09.491428145
CCAGTCA402250.09.443829145
Produced by FastQC (version 0.11.5)