FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6712122
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA53000.014.22619145
TATGCCG29000.013.999778145
ATCTCGT56900.013.633351145
CACCGCT169350.011.815578145
CGTATGC37350.011.258188145
ACCGCTG162300.011.212083145
TCGTATG42600.011.062031145
CGCTGAT142650.011.028566145
CTCGTAT48050.011.014394145
TCACCGC196800.010.793782145
CATCTCG77500.010.477253145
GTCACCG230750.010.273943145
AGTCACC257850.010.009537145
CCGCTGA168150.09.916583145
CAGTCAC300850.09.591006145
CGTCTGA520000.09.466677145
CCAGTCA305600.09.347037145
TCCAGTC325700.09.148611145
AACTCCA409500.09.1176815145
CTGATCA157700.09.056609145
Produced by FastQC (version 0.11.5)