FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6712122
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG11800.024.575329145
GTGGTCG71650.013.153717145
TGGTCGC62200.012.704495145
CGCCGTA32850.012.358734145
GCCGTAT33800.012.2258625145
CCGTATC34600.011.943184145
GGTCGCC71200.011.709522145
TCTCGGT119300.011.667615145
GAGTGTA252800.011.126946145
AGTGTAG238650.011.087988145
GTGTAGA226700.010.841004145
GATCTCG152100.010.581448145
TAGATCT184750.010.359549145
TCGCCGT47100.010.158845145
GTCGCCG53800.09.971737145
CGGTGGT98950.09.817713145
CGTATCA30600.09.713679145
AGAGTGT291050.09.589909145
GTAGATC248450.09.541796145
TAGGGAA446000.09.509164145
Produced by FastQC (version 0.11.5)