FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6458610
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG18450.028.685286145
GCCGTAT60000.014.378991145
CGCCGTA64050.013.5829735145
CGTATCA48850.013.505465145
TGGTCGC109400.013.187682145
CCGTATC58350.012.6733675145
GTGGTCG124950.011.720545145
GGTCGCC116100.011.5524145
GATCTCG252950.011.292608145
TAGATCT278750.011.235737145
GTCGCCG100700.011.015257145
CGGTGGT163900.010.571865145
GTGTAGA354900.010.540869145
GAGTGTA399850.010.480051145
TCGCCGT89500.010.449593145
GTATCAT64100.010.292386145
AGTGTAG371650.010.28038145
GTAGATC361500.09.826989145
AGAGTGT446800.09.816912145
TCTCGGT199150.09.79275145
Produced by FastQC (version 0.11.5)