FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6506132
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28350.013.553126145
CTCGTAT47350.012.707929145
TCTCGTA52000.012.129212145
ATCTCGT55450.011.897522145
GGTCAAT114600.011.323615145
CGTATGC36700.011.2596655145
TCGTATG40900.011.166933145
GTATGCC50100.010.85276145
GTCAATC101600.010.489151145
CCAGTCA317000.010.314162145
AGTGGTC166850.010.210766145
GTGGTCA162300.09.916334145
GTCACAG259800.09.906173145
CGTCTGA542350.09.717878145
AACTCCA417100.09.716018145
AGTCACA284300.09.281993145
GTCTGAA561550.09.269423145
CTGAACT507150.09.205897145
CAGTCAC308600.09.161896145
ACGTCTG585100.09.0821905145
Produced by FastQC (version 0.11.5)