FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8138603
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG56850.014.283745145
CGTATGC49450.013.9287405145
ATCTCGT75000.013.437179145
TATGCCG39900.011.992937145
TCTCGTA70700.011.588135145
CTCGTAT64150.010.736963145
GTATGCC65850.010.019364145
GTCACAA295300.09.968208145
CATCTCG98300.09.440847145
TCACAAG295650.09.343329145
AGGACAC214650.09.187398145
CTCCAGT464600.09.097958145
CGTCTGA635050.08.985067145
CACAAGG270650.08.920532145
TCCAGTC409450.08.782847145
AGTCACA350850.08.7825775145
GAACTCC544700.08.758366145
AACTCCA498500.08.7119665145
CCAGTCA384950.08.60729145
CAAGGAC261900.08.581846145
Produced by FastQC (version 0.11.5)